The behaviour of pectin-degrading enzymes in dense media

The behaviour of pectin-degrading enzymes in dense media

The behaviour of pectin-degrading enzymes in dense media

Pectins impacts plant biomass recalcitrance by affecting the ability of other cell wall components to access enzyme degradation. Their removal thus has a positive effect on the saccharification of pectin-rich biomass. Furthermore, the development of environmentally-friendly processes involves reducing water consumption and using high-solids loadings. The aim of this research is thus to study the behaviour of different pectin-degrading enzymes in the presence of a low (5%) to high (35%) solid citrus-peel loading. Two enzymes conditions were studied, with a pectin lyase being compared with a mixture of endopolygalacturonase and pectin methylesterase.

Pectin impacts in plant biomass recalcitrance by affecting the ability of other cell wall components to access enzymatic degradation. Their removal thus has a positive effect on the saccharification of pectin-rich biomass.
Furthermore, the development of environmentally-friendly processes involves reducing water consumption and using high-solids loadings. We therefore compared the action of a pectin lyase and an endopolygalacturonase/pectin methylesterase mixture on citrus peels by varying the amount of water from 95% to 65%.
We showed that reducing the water content decreases the efficiency of pectin-degrading enzymes, more significantly for those catalyzing hydrolysis (polygalacturonase) than for those catalyzing β-elimination (pectin lyase). In parallel, low-field NMR experiments highlighted that solid loading clearly affects water mobility, and that these mobility changes vary depending on the enzyme used. Indeed, pectin lyase leads to less pronounced changes than the endopolygalacturonase/pectin methylesterase mixture.

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Modification date : 11 September 2023 | Publication date : 26 December 2022 | Redactor : MW